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described25  (Addgene inc)


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    Structured Review

    Addgene inc described25
    Described25, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/described25/pcDNA3-sACE2v2%2E4(732)-IgG1+(Plasmid+%23154106)/pm39185275-225-7-11
    Average 93 stars, based on 7 article reviews
    described25 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Modulation of the pharmacokinetics of soluble ACE2 decoy receptors through glycosylation
    Article Snippet: .. The expression plasmid for sACE22.v2.4-IgG1 is previously described25 and deposited with Addgene (# 154106). .. Briefly, the coding sequence is ligated into the NheI-XhoI sites of pcDNA3.1(+) (Invitrogen) and encompasses human ACE2 (GenBank: NM_021804.1) amino acids (a.a.) M1-G732 fused via a 1 serine linker to human IgG1 Fc a.a. D221-K447 (nG1m1 isoallotype; GenBank: KY432415.1).

    Article Title: Modulation of the pharmacokinetics of soluble ACE2 decoy receptors through glycosylation.
    Article Snippet: .. The expression plasmid for sACE22.v2.4-IgG1 is previously described25 and deposited with Addgene (# 154106). .. Briefly, the coding sequence is ligated into the NheI-XhoI sites of pcDNA3.1(+) (Invitrogen) and encompasses human ACE2 (GenBank: NM_021804.1) amino acids (a.a.) M1-G732 fused via a 1 serine linker to human IgG1 Fc a.a. D221-K447 (nG1m1 isoallotype; GenBank: KY432415.1).

    Plasmid Preparation:

    Article Title: Modulation of the pharmacokinetics of soluble ACE2 decoy receptors through glycosylation
    Article Snippet: .. The expression plasmid for sACE22.v2.4-IgG1 is previously described25 and deposited with Addgene (# 154106). .. Briefly, the coding sequence is ligated into the NheI-XhoI sites of pcDNA3.1(+) (Invitrogen) and encompasses human ACE2 (GenBank: NM_021804.1) amino acids (a.a.) M1-G732 fused via a 1 serine linker to human IgG1 Fc a.a. D221-K447 (nG1m1 isoallotype; GenBank: KY432415.1).


    Article Title: Modulation of the pharmacokinetics of soluble ACE2 decoy receptors through glycosylation.
    Article Snippet: .. The expression plasmid for sACE22.v2.4-IgG1 is previously described25 and deposited with Addgene (# 154106). .. Briefly, the coding sequence is ligated into the NheI-XhoI sites of pcDNA3.1(+) (Invitrogen) and encompasses human ACE2 (GenBank: NM_021804.1) amino acids (a.a.) M1-G732 fused via a 1 serine linker to human IgG1 Fc a.a. D221-K447 (nG1m1 isoallotype; GenBank: KY432415.1).

    Article Title: TIGER: Single-step in vivo genome editing in a non-traditional rodent.
    Article Snippet: Transfected and mock control cells were collected 96 h post transfection, and total genomic DNA was extracted using Zymo Quick DNA Miniprep Plus Kit (Zymo Research, D4068). .. The AAV.sgTyr.Nme2Cas9.Tyr_sgRNA plasmid was generously provided by Dr. Jaime Rivera (Frederick National Laboratory for Cancer Research) and has been previously described25 (Addgene 119924). ..

    Transfection:


    Clone Assay:


    Bioprocessing:

    Article Title: TIGER: Single-step in vivo genome editing in a non-traditional rodent.
    Article Snippet: Transfected and mock control cells were collected 96 h post transfection, and total genomic DNA was extracted using Zymo Quick DNA Miniprep Plus Kit (Zymo Research, D4068). .. The AAV.sgTyr.Nme2Cas9.Tyr_sgRNA plasmid was generously provided by Dr. Jaime Rivera (Frederick National Laboratory for Cancer Research) and has been previously described25 (Addgene 119924). ..



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